Microplate Readers and Reader Accessories
Microplate readers are a type of spectrophotometer designed specifically for analyzing multiple samples in multi-well plates.
The readers detect light signals that result from biological, chemical, biochemical, or physical reactions. The signals are produced by, converted by, or transmitted through the samples and are measured by a detector. The detectors are usually photomultiplier tubes (PMT), which convert the released photons into electricity. The current is quantified by the reader’s software to produce the numerical result.
In some cases, samples are first excited by exposure to light of specific wavelengths. Filters or monochromators are used to select or restrict the wavelengths needed for emission or detection.
Single-mode microplate readers commonly measure absorbance, fluorescence, or luminescence. Multi-mode plate readers may measure absorbance, fluorescence, and luminescence with options for fluorescence polarization (FP). They may also include lasers for time-resolved fluorescence (TRF), TR-FRET, Amplified Luminescent Proximity Homogeneous Assays (ALPHA), and cell imaging.
Features may include:
- Monochromator- or filter-based wavelength selection
- Visible only or UV/Vis spectrum, fluorescence, and luminescence
- Multiple photomultiplier tubes (PMTs) for simultaneous assays
- Temperature and CO2/O2controls
- Modular design for adding functionality
Microplate readers are used for high-throughput screening (HTS), biomarker assays, nucleic acid and protein quantification, kinetics, ELISA assays, homogeneous time resolved fluorescence (HTRF) for measuring cytokines, bioluminescence resonance energy transfer (BRET), and other techniques.
Revvity Health Sciences Inc HTRF Human Phospho-TYK2 (Tyr1054/1055) Detection Kit, 10,000 Assay Points
HTRF Human Phospho-TYK2 (Tyr1054/1055) Detection Kit, 10,000 Assay Points
Revvity Health Sciences Inc HTRF Human Total HSP27 Detection Kit, 10,000 Assay Points
HTRF Human Total HSP27 Detection Kit, 10,000 Assay Points
Revvity Health Sciences Inc HTRF Total ALK Detection Kit, Control Lysate
HTRF Total ALK Detection Kit, Control Lysate
Revvity Health Sciences Inc HTRF Human RIG-1 Binding Kit Standard
HTRF Human RIG-1 Binding Kit Standard
Revvity Health Sciences Inc HTRF CD32a (131R) Cellular Binding Kit, 1,000 Assay Points
HTRF CD32a (131R) Cellular Binding Kit, 1,000 Assay Points
Molecular Devices Fluorescence Polarization Filter, 700 nm, 60 nm, Horizontal
This fluorescence polarization filter is configured for near-infrared polarized fluorescence measurements and is optimized for a 700 nm center wavelength with a 60 nm bandwidth. It installs in a reader filter slot to enable polarization-based assays and filter-based detection in compatible multi-mode microplate readers.
- Provides 700 nm center wavelength with 60 nm bandwidth.
- Polarized horizontal orientation for fluorescence polarization assays.
- Installs in a standard filter slot or filter holder for multi-mode readers.
- Optimizes sensitivity for near-IR fluorophores and polarization-based detection.
- Store in a dust-free environment and avoid touching optical surfaces.
Revvity Health Sciences Inc HTRF Human Phospho-JAK1 (Tyr1034/1035) Detection Kit, 500 Assay Points
HTRF Human Phospho-JAK1 (Tyr1034/1035) Detection Kit, 500 Assay Points
Revvity Health Sciences Inc HTRF Human Total CDK7 Detection Kit, 500 Assay Points
HTRF Human Total CDK7 Detection Kit, 500 Assay Points
Molecular Devices Fluorescence Polarization Filter, 590 nm, 20 nm, Vertical
Molecular Devices fluorescence polarization filter designed for fluorescence polarization assays in compatible microplate readers. The filter has a 590 nm center wavelength, a 20 nm full-width bandwidth, and is supplied with a mounting holder for installation.
- 590 nm center wavelength, 20 nm bandwidth.
- Polarized vertical orientation.
- Supplied with mounting holder for installation.
- Optimized for fluorescence polarization measurements.
- Suitable for use in compatible microplate and ELISA readers.
Molecular Devices Fluorescence Polarization Filter, 635 nm, 35 nm, Vertical
Molecular Devices fluorescence polarization filter with holder designed for fluorescence polarization assays and FP-compatible detection on compatible multi-mode microplate readers. The filter is specified for a 635 nm center wavelength with a 35 nm bandwidth and is polarized to enable fluorescence polarization measurements.
- Designed for fluorescence polarization assays and FP detection modes.
- Center wavelength 635 nm with 35 nm bandwidth.
- Polarized to enable measurement of fluorescence anisotropy.
- Includes holder for installation into compatible filter assemblies.
- Intended as an accessory for multi-mode microplate readers that accept replaceable filters.
Molecular Devices Absorbance Filter, 350 nm, 10 nm Bandwidth
Molecular Devices 350 nm absorbance filter with 10 nm bandwidth provides narrowband absorbance detection for microplate assays. It is sold as a replaceable filter accessory for compatible absorbance microplate readers and is intended to improve selectivity for wavelength-specific absorbance measurements.
- Center wavelength: 350 nm
- Bandwidth (full width at half maximum): 10 nm
- Filter type: Absorbance
- Intended application: Narrowband absorbance measurements in microplate assays
- Compatibility: Designed for use in compatible absorbance microplate reader filter assemblies
Revvity Health Sciences Inc HTRF Total MSH3 Detection Kit, Control Lysate
HTRF Total MSH3 Detection Kit, Control Lysate
Molecular Devices Absorbance Filter, 260 nm, 5.5 nm Bandwidth
The Molecular Devices 260 nm absorbance filter (5.5 nm bandwidth) is an optical bandpass accessory designed for use with compatible FilterMax series multimode microplate readers. It provides a narrow UV transmission centered at 260 nm for precise absorbance measurements of nucleic acids and other UV-absorbing analytes.
- Provides narrow bandpass transmission centered at 260 nm.
- 5.5 nm bandwidth for selective UV absorbance measurements.
- Suitable for nucleic acid quantification and other UV-absorbing samples.
- Designed for easy installation in modular filter holders.
- Improves measurement specificity by reducing out-of-band light.