Microplate Readers and Reader Accessories
Microplate readers are a type of spectrophotometer designed specifically for analyzing multiple samples in multi-well plates.
The readers detect light signals that result from biological, chemical, biochemical, or physical reactions. The signals are produced by, converted by, or transmitted through the samples and are measured by a detector. The detectors are usually photomultiplier tubes (PMT), which convert the released photons into electricity. The current is quantified by the reader’s software to produce the numerical result.
In some cases, samples are first excited by exposure to light of specific wavelengths. Filters or monochromators are used to select or restrict the wavelengths needed for emission or detection.
Single-mode microplate readers commonly measure absorbance, fluorescence, or luminescence. Multi-mode plate readers may measure absorbance, fluorescence, and luminescence with options for fluorescence polarization (FP). They may also include lasers for time-resolved fluorescence (TRF), TR-FRET, Amplified Luminescent Proximity Homogeneous Assays (ALPHA), and cell imaging.
Features may include:
- Monochromator- or filter-based wavelength selection
- Visible only or UV/Vis spectrum, fluorescence, and luminescence
- Multiple photomultiplier tubes (PMTs) for simultaneous assays
- Temperature and CO2/O2controls
- Modular design for adding functionality
Microplate readers are used for high-throughput screening (HTS), biomarker assays, nucleic acid and protein quantification, kinetics, ELISA assays, homogeneous time resolved fluorescence (HTRF) for measuring cytokines, bioluminescence resonance energy transfer (BRET), and other techniques.
Revvity Health Sciences Inc HTRF Human Total KRAS Detection Kit, 50,000 Assay Points
HTRF Human Total KRAS Detection Kit, 50,000 Assay Points
Alkali Scientific ASI™ 6-Well Confocal Plate (35m), Clear, Adhesion Type, Sterile, 4/Cs, PS/Flux, Treated (Positive-Charged/ Surface Coated), 50/Cs, 22 x 22 x 0.13mm, Hole 1.33mm
ASI™ confocal products allow researchers to acquire high resolution microscopic images of cells in 35 mm culture dishes or 6 well plates. Low auto-fluorescence glass and FLux were used, ideal for uses of these products are confocal microscopy, phase contrast microscopy, live cell imaging and micro-manipulations. Surface materials for confocal products: Glass: naturally charged, good growth surface for adherent cellsFLux: surface modified plastic film (188 μm thick), optimal for confocal microscopy ▪ Bottom materials: Glass/FLux (no surface treatment for glass bottom)▪ Dish color: Clear▪ Dish size: 35 Ø▪ Plate size: 6well Plate▪ Hole size: 13 Ø / 20 Ø / 25Ø▪ Non – pyrogenic▪ Non – cytotoxic▪ DNase / RNase – free▪ Human DNA – free
Revvity Health Sciences Inc HTRF Human IRF5 Detection Kit, 50,000 Assay Points
HTRF Human IRF5 Detection Kit, 50,000 Assay Points
Revvity Health Sciences Inc HTRF Human Total VAV1 Detection Kit, 50,000 Assay Points
HTRF Human Total VAV1 Detection Kit, 50,000 Assay Points
Molecular Devices Fluorescence Polarization Filter, 360 nm, 35 nm, Horizontal
Fluorescence polarization filter slide with integrated holder for microplate readers. Optimized for excitation at 360 nm with a 35 nm optical bandwidth and horizontal polarization orientation, this filter is used in fluorescence polarization assays to measure molecular binding and rotational mobility.
- Designed for fluorescence polarization measurements
- Optimized excitation wavelength 360 nm with 35 nm bandwidth
- Horizontal polarization orientation for emission analysis
- Includes an integrated holder for secure installation in filter slides
- Compatible with multi-mode microplate readers that accept filter slides
Revvity Health Sciences Inc AlphaLISA SureFire Ultra Human Total TIE2 Detection Kit, 100 Assay Points
AlphaLISA SureFire Ultra Human Total TIE2 Detection Kit, 100 Assay Points
Revvity Health Sciences Inc HTRF Human Total DDR1 Detection Kit, 10,000 Assay Points
HTRF Human Total DDR1 Detection Kit, 10,000 Assay Points
Revvity Health Sciences Inc HTRF Human IL-1β High Performance Detection Kit, 10,000 Assay Points
HTRF Human IL-1β High Performance Detection Kit, 10,000 Assay Points
INVISION BIORESOURCES Rod Outer Segment (ROS), Bovine
This item has a minimum order quantity of 50 per supplier requirements
Prepared under dim red light from dark-adapted (DA) /dark-dissected (DD) bovine retina
Revvity Health Sciences Inc HTRF MDM2 Binding Kit, 500 Assay Points
HTRF MDM2 Binding Kit, 500 Assay Points
Revvity Health Sciences Inc HTRF Human & Mouse Phospho-SMAD2 (Ser465/467) Detection Kit, 50,000 Assay Points
HTRF Human & Mouse Phospho-SMAD2 (Ser465/467) Detection Kit, 50,000 Assay Points
Revvity Health Sciences Inc HTRF Human Androgen Receptor Detection Kit, 500 Assay Points
HTRF Human Androgen Receptor Detection Kit, 500 Assay Points
Revvity Health Sciences Inc HTRF CHO HCP Detection Kit, 10,000 Assay Points
HTRF CHO HCP Detection Kit, 10,000 Assay Points